Gut Bacterial Flora of Open Nested Honeybee, Apis florea

Honeybees are eusocial insects with close interaction their surrounding environment. Gut microbiota in honeybees play a significant role host health, biology, and behavior the Apis florea , wild bee, is most primitive among all indigenous to Indian subcontinent. Previous reports on reared honeybee species provide information gut microbiome. No such studies reported of species. This study aimed at studying microbiome species, A. . The analysis identification bacteria employing culture-based culture-independent methods. Cultured were identified characterized by MALDI-TOF MS 16S rRNA sequencing. A comprehensive non-culturable performed amplicon next-generation approach splits into four bacterial phyla, families, 10 genera major. dominant taxa belonged family Enterobacteriaceae (79.47%), Lactobacillaceae (12.75%), Oxalobacteraceae (7.45%), Nocardiaceae (0.13%). prevailing Enterobacter, Lactobacillus, Escherichia-Shigella, Massilia, Klebsiella, Citrobacter, Pantoea, Serratia, Rhodococcus Morganella genera, belonging phyla Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes. observed occurrence few that not previously for other genus, making this investigation highly relevant regard bee